Software:Article Title: Protocol for affinity purification-mass spectrometry interactome profiling in larvae of Drosophila melanogaster.
Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Python Python Software Foundation https://www.python.org/; RRID:SCR_008394 Anaconda Anaconda, Inc. https://www.anaconda.com/ Unipressed https://multimeric.github. io/Unipressed/ Other 6oz Square bottom Drosophila bottles Genesee Scientific Cat# 32-130 Flugs - plastic fly bottles, Drosophila closures Genesee Scientific Cat# 49-100 Fisherbrand Premium microcentrifuge tubes, 1.5 mL Fisher Scientific Cat# 05-408-129 Eppendorf centrifuge 5424 R Fisher Scientific Cat# 05-413-947PM; RRID:SCR_019852 DynaMag - Spin magnet Thermo Fisher Scientific Cat# 12320D Corning PYREX Dounce homogenizer, 7 mL Fisher Scientific Cat# 11-850-51 Thermo Scientific Labquake tube shaker/rotator Fisher Scientific Cat# 13-687-12Q Flystuff Embryo Collection Cage-Mini Genesee Scientific Cat# 59-105 Olympus Plastics 24–112, 200 mL round gel tip, 0.57 mm racked Genesee Scientific Cat# 24-112 BioPureSPN C18 spin column The Nest Group Cat# HMM S18R Acclaim PepMap 100 C18 HPLC column, 70 mm, trap column Thermo Fisher Scientific Cat# 164946 Acclaim PepMap 100 C18 HPLC column, 500 mm, analytical column Thermo Fisher Scientific Cat# 164942 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific FLAG lysis buffer Reagent Final concentration Volume 5 M NaCl 100 mM 1 mL 1 M Tris-HCl pH 7.3 50 mM 2.5 mL 10% IGEPAL CA-630 1% 5 mL Milli-Q water N/A 41.5 mL Total N/A 50 mL The solution can be stored at 4 C for up to 1 week. ..
High Performance Liquid Chromatography:Article Title: Protocol for affinity purification-mass spectrometry interactome profiling in larvae of Drosophila melanogaster.
Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Python Python Software Foundation https://www.python.org/; RRID:SCR_008394 Anaconda Anaconda, Inc. https://www.anaconda.com/ Unipressed https://multimeric.github. io/Unipressed/ Other 6oz Square bottom Drosophila bottles Genesee Scientific Cat# 32-130 Flugs - plastic fly bottles, Drosophila closures Genesee Scientific Cat# 49-100 Fisherbrand Premium microcentrifuge tubes, 1.5 mL Fisher Scientific Cat# 05-408-129 Eppendorf centrifuge 5424 R Fisher Scientific Cat# 05-413-947PM; RRID:SCR_019852 DynaMag - Spin magnet Thermo Fisher Scientific Cat# 12320D Corning PYREX Dounce homogenizer, 7 mL Fisher Scientific Cat# 11-850-51 Thermo Scientific Labquake tube shaker/rotator Fisher Scientific Cat# 13-687-12Q Flystuff Embryo Collection Cage-Mini Genesee Scientific Cat# 59-105 Olympus Plastics 24–112, 200 mL round gel tip, 0.57 mm racked Genesee Scientific Cat# 24-112 BioPureSPN C18 spin column The Nest Group Cat# HMM S18R Acclaim PepMap 100 C18 HPLC column, 70 mm, trap column Thermo Fisher Scientific Cat# 164946 Acclaim PepMap 100 C18 HPLC column, 500 mm, analytical column Thermo Fisher Scientific Cat# 164942 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific FLAG lysis buffer Reagent Final concentration Volume 5 M NaCl 100 mM 1 mL 1 M Tris-HCl pH 7.3 50 mM 2.5 mL 10% IGEPAL CA-630 1% 5 mL Milli-Q water N/A 41.5 mL Total N/A 50 mL The solution can be stored at 4 C for up to 1 week. ..
Mass Spectrometry:Article Title: Protocol for affinity purification-mass spectrometry interactome profiling in larvae of Drosophila melanogaster.
Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Python Python Software Foundation https://www.python.org/; RRID:SCR_008394 Anaconda Anaconda, Inc. https://www.anaconda.com/ Unipressed https://multimeric.github. io/Unipressed/ Other 6oz Square bottom Drosophila bottles Genesee Scientific Cat# 32-130 Flugs - plastic fly bottles, Drosophila closures Genesee Scientific Cat# 49-100 Fisherbrand Premium microcentrifuge tubes, 1.5 mL Fisher Scientific Cat# 05-408-129 Eppendorf centrifuge 5424 R Fisher Scientific Cat# 05-413-947PM; RRID:SCR_019852 DynaMag - Spin magnet Thermo Fisher Scientific Cat# 12320D Corning PYREX Dounce homogenizer, 7 mL Fisher Scientific Cat# 11-850-51 Thermo Scientific Labquake tube shaker/rotator Fisher Scientific Cat# 13-687-12Q Flystuff Embryo Collection Cage-Mini Genesee Scientific Cat# 59-105 Olympus Plastics 24–112, 200 mL round gel tip, 0.57 mm racked Genesee Scientific Cat# 24-112 BioPureSPN C18 spin column The Nest Group Cat# HMM S18R Acclaim PepMap 100 C18 HPLC column, 70 mm, trap column Thermo Fisher Scientific Cat# 164946 Acclaim PepMap 100 C18 HPLC column, 500 mm, analytical column Thermo Fisher Scientific Cat# 164942 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific FLAG lysis buffer Reagent Final concentration Volume 5 M NaCl 100 mM 1 mL 1 M Tris-HCl pH 7.3 50 mM 2.5 mL 10% IGEPAL CA-630 1% 5 mL Milli-Q water N/A 41.5 mL Total N/A 50 mL The solution can be stored at 4 C for up to 1 week. ..
Lysis:Article Title: Protocol for affinity purification-mass spectrometry interactome profiling in larvae of Drosophila melanogaster.
Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Python Python Software Foundation https://www.python.org/; RRID:SCR_008394 Anaconda Anaconda, Inc. https://www.anaconda.com/ Unipressed https://multimeric.github. io/Unipressed/ Other 6oz Square bottom Drosophila bottles Genesee Scientific Cat# 32-130 Flugs - plastic fly bottles, Drosophila closures Genesee Scientific Cat# 49-100 Fisherbrand Premium microcentrifuge tubes, 1.5 mL Fisher Scientific Cat# 05-408-129 Eppendorf centrifuge 5424 R Fisher Scientific Cat# 05-413-947PM; RRID:SCR_019852 DynaMag - Spin magnet Thermo Fisher Scientific Cat# 12320D Corning PYREX Dounce homogenizer, 7 mL Fisher Scientific Cat# 11-850-51 Thermo Scientific Labquake tube shaker/rotator Fisher Scientific Cat# 13-687-12Q Flystuff Embryo Collection Cage-Mini Genesee Scientific Cat# 59-105 Olympus Plastics 24–112, 200 mL round gel tip, 0.57 mm racked Genesee Scientific Cat# 24-112 BioPureSPN C18 spin column The Nest Group Cat# HMM S18R Acclaim PepMap 100 C18 HPLC column, 70 mm, trap column Thermo Fisher Scientific Cat# 164946 Acclaim PepMap 100 C18 HPLC column, 500 mm, analytical column Thermo Fisher Scientific Cat# 164942 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific FLAG lysis buffer Reagent Final concentration Volume 5 M NaCl 100 mM 1 mL 1 M Tris-HCl pH 7.3 50 mM 2.5 mL 10% IGEPAL CA-630 1% 5 mL Milli-Q water N/A 41.5 mL Total N/A 50 mL The solution can be stored at 4 C for up to 1 week. ..
Concentration Assay:Article Title: Protocol for affinity purification-mass spectrometry interactome profiling in larvae of Drosophila melanogaster.
Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Python Python Software Foundation https://www.python.org/; RRID:SCR_008394 Anaconda Anaconda, Inc. https://www.anaconda.com/ Unipressed https://multimeric.github. io/Unipressed/ Other 6oz Square bottom Drosophila bottles Genesee Scientific Cat# 32-130 Flugs - plastic fly bottles, Drosophila closures Genesee Scientific Cat# 49-100 Fisherbrand Premium microcentrifuge tubes, 1.5 mL Fisher Scientific Cat# 05-408-129 Eppendorf centrifuge 5424 R Fisher Scientific Cat# 05-413-947PM; RRID:SCR_019852 DynaMag - Spin magnet Thermo Fisher Scientific Cat# 12320D Corning PYREX Dounce homogenizer, 7 mL Fisher Scientific Cat# 11-850-51 Thermo Scientific Labquake tube shaker/rotator Fisher Scientific Cat# 13-687-12Q Flystuff Embryo Collection Cage-Mini Genesee Scientific Cat# 59-105 Olympus Plastics 24–112, 200 mL round gel tip, 0.57 mm racked Genesee Scientific Cat# 24-112 BioPureSPN C18 spin column The Nest Group Cat# HMM S18R Acclaim PepMap 100 C18 HPLC column, 70 mm, trap column Thermo Fisher Scientific Cat# 164946 Acclaim PepMap 100 C18 HPLC column, 500 mm, analytical column Thermo Fisher Scientific Cat# 164942 Orbitrap Fusion mass spectrometer Thermo Fisher Scientific FLAG lysis buffer Reagent Final concentration Volume 5 M NaCl 100 mM 1 mL 1 M Tris-HCl pH 7.3 50 mM 2.5 mL 10% IGEPAL CA-630 1% 5 mL Milli-Q water N/A 41.5 mL Total N/A 50 mL The solution can be stored at 4 C for up to 1 week. ..
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